Elizabeth M. Topp, Ph.D. Head, Department of Industrial and Physical Pharmacy Dane O. Kildsig Chair in Industrial and Physical Pharmacy Phone: (765) 494-1450 Fax: (765) 494-6545 E-mail: topp@purdue.edu Full directory listing

Specialization: Chemical and physical stability of peptide and protein drugs in solid state. Mechanisms of chemical degradation reactions in amorphous solids, higher resolution methods for protein conformation in solids, and improved stability of biotech drug products.

Education

Research: Chemical and physical stability of peptide and protein drugs in solid state. Mechanisms of chemical degradation reactions in amorphous solids, higher resolution methods for protein conformation in solids, and improved stability of biotech drug products.

Professor Topp's research focuses on the chemical and physical stability of protein drugs, with particular emphasis on chemical degradation reactions in the amorphous solid state. Currently active research projects address (i) the effects of secondary structure in deamidation in the Fc portion of an IgG, (ii) the development of hydrogen/deuterium exchange methods for examining protein structure and excipient interactions in amorphous solids,(iii) the controlled release of immunomodulating peptides from PLGA matrices in type-1 diabetes, and (iv) mechanisms of degradation of phenylephrine and cetirizine in the solid state.

Representative Publications

Reversibility and regioselectivity of thiol/disulfide exchange in lyophilized solids, L. Zhang, T.D. Williams and E.M. Topp. Journal of Pharmaceutical Sciences, 98/9: 3312-3318, 2009.

A computational molecular design framework for crosslinked polymer networks, J.C. Eslick, Q. Ye, J. Park, E.M. Topp, P. Spencer, K.V. Camarda. Computers and Chemical Engineering, 33: 954-963, 2009.

Effect of photo-initiators on the in vitro performance of a dentin adhesive exposed to simulated oral environment. Qiang Ye, Yong Wang, Elizabeth Topp and Paulette Spencer. Dental Materials, 25/4: 452-458, 2009.

Effect of secondary structure on deamidation in the Fc fragment of an IgG1 monoclonal antibody. Sandipan Sinha, Lei Zhang, Shaofeng Duan, Todd Williams, Josef Vlasak, Roxana Ionescu and Elizabeth Topp. Protein Science, 18/8: 1573-1584, 2009. DOI: 10.1002/pro.173.

Comparison of LC and LC/MS methods for quantifying N-glycosylation in recombinant IgGs. Sandipan Sinha, Gary Pipes, Elizabeth Topp, Pavel V. Bondarenko, Michael Treuheit, and Himanshu S. Gadgil. Journal of the American Society for Mass Spectrometry, 19/11: 1643-54, 2008. DOI: 10.1016/j.jasms. 2008.07.004.

Protein conformation in amorphous solids by FTIR and by hydrogen/deuterium exchange with mass spectrometry. S. Sinha, Y. Li, T.D. Williams and E.M. Topp, Biophysical Journal, 95/12: 5951-5961, 2008. DOI:10.1529/biophysj.108.139899. PMCID: PMC2599811

Effects of excipients on protein conformation in lyophilized solids by hydrogen/deuterium exchange with mass spectrometry. Y. Li, T. D. Williams and E. M. Topp, Pharmaceutical Research, 25/2: 259-267, 2008.

Chemical degradation of peptides and proteins in PLGA: A review of reactions and mechanisms (invited minireview). M.L. Houchin and E.M. Topp, Journal of Pharmaceutical Sciences, 97/7: 2395-2404, 2008. DOI: 10.1002/jps.21176

Trehalose and calcium exert site-specific effects on calmodulin conformation in amorphous solids. Y. Li, T.D. Williams, R.L. Schowen and E.M. Topp, Biotechnology and Bioengineering, 97/6: 1650-1653, 2007.

Effect of excipients on PLGA film degradation and on the stability of an incorporated peptide. M.L. Houchin, S.A. Neuenswander and E.M. Topp, Journal of Controlled Release, 117: 413-420, 2007.

Curriculum Vitae

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